| مشخصات مقاله | |
| ترجمه عنوان مقاله | عنوان فارسی مقاله: WGBS همراه با تجزیه و تحلیل RNA-seq نشان داد که Dnmt1 بر اصلاح متیلاسیون و تغییرات بیان ژن در طی انجماد تخمک موش تأثیر می گذارد. |
| عنوان انگلیسی مقاله | WGBS combined with RNA-seq analysis revealed that Dnmt1 affects the methylation modification and gene expression changes during mouse oocyte vitrification |
| انتشار | مقاله سال 2022 |
| تعداد صفحات مقاله انگلیسی | 11 صفحه |
| هزینه | دانلود مقاله انگلیسی رایگان میباشد. |
| پایگاه داده | نشریه الزویر |
| نوع نگارش مقاله |
مقاله پژوهشی (Research Article) |
| مقاله بیس | این مقاله بیس نمیباشد |
| نمایه (index) | Scopus – Master Journals List – JCR – Medline |
| نوع مقاله | ISI |
| فرمت مقاله انگلیسی | |
| ایمپکت فاکتور(IF) |
2.740 در سال 2020 |
| شاخص H_index | 133 در سال 2020 |
| شاخص SJR | 0.816 در سال 2020 |
| شناسه ISSN | 0093-691X |
| شاخص Quartile (چارک) | Q1 در سال 2020 |
| فرضیه | ندارد |
| مدل مفهومی | ندارد |
| پرسشنامه | ندارد |
| متغیر | ندارد |
| رفرنس | دارد |
| رشته های مرتبط | زیست شناسی، پزشکی |
| گرایش های مرتبط | ژنتیک، میکروبیولوژی، علوم سلولی و مولکولی، بیولوزی تولید مثل |
| نوع ارائه مقاله |
ژورنال |
| مجله | تریوژنولوژی – Theriogenology |
| دانشگاه | Centre of Reproductive Medicine, Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia, China |
| کلمات کلیدی | انجماد تخمک، متیلاسیون DNA، بیان ژن، شبکه تنظیم ژن، توانایی رشد، پتانسیل لقاح |
| کلمات کلیدی انگلیسی | Oocyte cryopreservation – DNA methylation – Gene expression – Gene regulatory network – Developmental ability – Fertilization potential |
| شناسه دیجیتال – doi |
https://doi.org/10.1016/j.theriogenology.2021.09.032 |
| کد محصول | E15728 |
| وضعیت ترجمه مقاله | ترجمه آماده این مقاله موجود نمیباشد. میتوانید از طریق دکمه پایین سفارش دهید. |
| دانلود رایگان مقاله | دانلود رایگان مقاله انگلیسی |
| سفارش ترجمه این مقاله | سفارش ترجمه این مقاله |
| فهرست مطالب مقاله: |
| Abstract Keywords Introduction Materials and methods Results Discussion Declaration of competing interest CRediT authorship contribution statement CRediT authorship contribution statement Acknowledgments Appendix A. Supplementary data References |
| بخشی از متن مقاله: |
| Abstract Understanding the molecular level changes of oocyte cryopreservation and the subsequent warming process is essential for improving the oocyte cryopreservation technologies. Here, we collected the mature metaphase II (MII) oocytes from mice and vitrified. After thawing, single-cell whole-genome bisulphite sequencing (scWGBS) and single-cell RNA sequencing (scRNA-seq) were used to investigate the molecular attributes of this process. Compared to the fresh oocytes, the vitrified oocytes had lower global methylation and gene expression levels, and 1426 genes up-regulated and 3321 genes downregulated. The 1426 up-regulated differentially expressed genes (DEGs) in the vitrified oocytes were mainly associated with the histone ubiquitination, while the 3321 down-regulated genes were mainly enriched in the mitochondrion organisation and ATP metabolism processes. The differentially methylated regions (DMRs) were mainly located in promoter, intron and exon region of genes, and the length of DMRs in the vitrified oocytes were also significantly lower than that of the fresh oocytes. Notably, there were no significant difference in the expression levels of DNA demethylases (Tet1, Tet2 and Tet3) and methyltransferases (Dnmt3a and Dnmt3b) between two treatments of oocytes. However, Dnmt1 and kcnq1ot1, which are responsible for maintaining DNA methylation, were significantly down regulated in the vitrified oocytes. Gene regulatory network (GRN) analysis showed the Dnmt1 and kcnq1ot1 play a core role in regulating methylation and expression levels of downstream genes. Moreover, some genes associated with oocyte quality were significantly down-regulated in the vitrified oocytes. The present data provides a new perspective for understanding the impact of vitrification on oocytes. |