| مشخصات مقاله | |
| ترجمه عنوان مقاله | اثر ضد توموری OP-B بر سرطان تخمدان در شرایط in vitro و in vivo و مکانیسم آن: بررسی با استفاده از تجزیه و تحلیل مبتنی بر داروشناسی شبکه |
| عنوان انگلیسی مقاله | The anti-tumor effect of OP-B on ovarian cancer in vitro and in vivo, and its mechanism: An investigation using network pharmacology-based analysis |
| انتشار | مقاله سال 2022 |
| تعداد صفحات مقاله انگلیسی | 11 صفحه |
| هزینه | دانلود مقاله انگلیسی رایگان میباشد. |
| پایگاه داده | نشریه الزویر |
| نوع نگارش مقاله |
مقاله پژوهشی (Research Article) |
| مقاله بیس | این مقاله بیس نمیباشد |
| نمایه (index) | Scopus – Master Journals List – JCR – Medline |
| نوع مقاله | ISI |
| فرمت مقاله انگلیسی | |
| ایمپکت فاکتور(IF) |
4.360 در سال 2020 |
| شاخص H_index | 192 در سال 2020 |
| شاخص SJR | 0.885 در سال 2020 |
| شناسه ISSN | 0378-8741 |
| شاخص Quartile (چارک) | Q1 در سال 2020 |
| فرضیه | ندارد |
| مدل مفهومی | ندارد |
| پرسشنامه | ندارد |
| متغیر | ندارد |
| رفرنس | دارد |
| رشته های مرتبط | پزشکی، داروسازی |
| گرایش های مرتبط | ایمنی شناسی پزشکی، خون و آنکولوژی، داروشناسی |
| نوع ارائه مقاله |
ژورنال |
| مجله | مجله علوم اجتماعی آسیایی – Asian Journal of Social Science |
| کلمات کلیدی | اوفیوپوگونین B ، سرطان تخمدان، داروسازی شبکه، مسیر STAT3 |
| کلمات کلیدی انگلیسی | Ophiopogonin B – Ovarian cancer – Network pharmacology – STAT3 pathway |
| شناسه دیجیتال – doi |
https://doi.org/10.1016/j.jep.2021.114706 |
| کد محصول | E15644 |
| وضعیت ترجمه مقاله | ترجمه آماده این مقاله موجود نمیباشد. میتوانید از طریق دکمه پایین سفارش دهید. |
| دانلود رایگان مقاله | دانلود رایگان مقاله انگلیسی |
| سفارش ترجمه این مقاله | سفارش ترجمه این مقاله |
| فهرست مطالب مقاله: |
| Abstract Graphical abstract Keywords List of abbreviation Introduction Materials and methods Results Discussion Conclusion Funding CRediT authorship contribution statement Declaration of competing interest References |
| بخشی از متن مقاله: |
| ABSTRACT Ethnopharmacological relevance: Maidong (Liliaceae) is used as a yin-nourishing medication for the treatment of cardiovascular disease, inflammation, and assistant cancer chemotherapy in the clinic. Ophiopogonin B (OP-B), a major saponin extracted from Maidong, is reported to have potential antitumor activities against various human cancers. However, the effects of OP-B on human ovarian cancer (OC) and the potential mechanisms of action are yet elusive. Aim of the study: In this study, we aimed to explore the potential molecular mechanisms of OP-B in the treatment of OC using network pharmacology. In vivo and in vitro experiments were conducted to further verify the therapeutic effects of OP-B on OC. Materials and methods: To investigate the functions of OP-B against OC holistically, the related targets of OP-B and OC were each predicted based on four public databases. Subsequently, the identified PPI network was constructed to detect the hub potential targets. In addition, GO and KEGG enrichment analysis were applied by Metascape database. Furthermore, we simultaneously investigated the anticancer effects of OP-B on SKOV3 and A2780 human ovarian cancer cells using a cell viability assay, transwell assay, and an image-based cytometric assay. The quantitative real-time PCR and western-blot assay were used to validate the RNA and protein levels of target genes in OP-B treated OC cells. At last, SKOV3-bearing BALB/c nude mice were applied to observe the effectiveness and toxicity of OP-B. Results: Through network pharmacological analysis, OP-B was found to play a critical role in OC via multiple targets and pathways, especially the STAT3 signaling pathways. In addition, in vitro experiments found OP-B suppressed SKOV3 and A2780 cells proliferation in a time and concentration dependent manner, and markedly impaired cancer cell migration. Flow cytometry analysis revealed that OP-B significantly increased early and late apoptosis, induced G2/M phase cell cycle arrest in SKOV3 cells and G0/G1 phase cell cycle arrest in A2780 cells |