مقاله انگلیسی رایگان در مورد مهندسی متابولیسم کارخانه های آنتی بیوتیک – CellPress 2015

Actinomycetes are excellent sources for novel bioactive compounds, which serve as potential drug candidates for antibiotics development. While industrial efforts to find and develop novel antimicrobials have been severely reduced during the past two decades, the increasing threat of multidrug-resistant pathogens and the development of new technologies to find and produce such compounds have again attracted interest in this field. Based on improvements in whole-genome sequencing, novel methods have been developed to identify the secondary metabolite biosynthetic gene clusters by genome mining, to clone them, and to express them in heterologous hosts in much higher throughput than before. These technologies now enable metabolic engineering approaches to optimize production yields and to directly manipulate the pathways to generate modified products.

Actinomycetes as sources for novel drugs

For more than 70 years, actinomycetes (order Actinomycetales) have been recognized as important sources for bioactive natural compounds. From the roughly 18 000 known bioactive bacterial compounds, more than 10 000 were described from bacteria of the actinomycete genus Streptomyces [1]. Many well-known antibiotics, such as tetracycline, erythromycin, vancomycin, and streptomycin, originate from the secondary metabolism of actinomycetes. Beyond antibiotics, other medically useful natural products that were isolated from this group of bacteria include the immunosuppressant rapamycin, the anticancer agents doxorubicin and bleomycin, the anthelmintic avermectin, and the antifungal compound nystatin.